Collection of Czechoslovak Chemical Communications - digital archive 

Abstract

The biosynthesis of lactose (O-β-D-galactopyranosyl-(1 → 4)-D-glucopyranose was investigated in a reaction catalyzed by lactosynthetase (UDP-D-galactose: D-glucose-4-β-D-galactosyltransferase) isolated from milk. The reaction donor in this reaction was UDP-D-galactose, the acceptor D-glucose or its monodeoxy derivatives. As found, monodeoxyglucose - excepting 4-deoxy-D-glucose - enter the transglycosylation reaction of biosynthesis of lactose. The affinity of the enzyme and the rate of biosynthesis of lactose and its deoxy derivatives decrease in the following order: lactose, 2^G-deoxylactose, 6^G-deoxylactose, 3^G-deoxylactose.