The preparation and metabolic fate of tritiated N<sup>α</sup>-acetyl[2-O-methyltyrosine]oxytocin - An inhibitor of the uterotonic action of oxytocin

Bojanovska, Vera; Barth, Tomislav; Černý, Bohuslav; Hauzer, Karel; Jošt, Karel

doi:10.1135/cccc19792702

CCCC > Archive > 1979, Volume 44 > Issue 9 > p. 2702

The preparation and metabolic fate of tritiated N^α-acetyl[2-O-methyltyrosine]oxytocin - An inhibitor of the uterotonic action of oxytocin

Vera Bojanovska, Tomislav Barth^a, Bohuslav Černý^b, Karel Hauzer^a and Karel Jošt^a

^a Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 166 10 Prague 6
^b Isotope Laboratory of Biological Institutes, Czechoslovak Academy of Sciences, 142 20 Prague 4

Abstract

An inhibitor of the uterotonic action of oxytocin-[CH₃CO-³H] [2-O-methyltyrosine]oxytocin - having biological activity and a specific radioactivity of 3-7 Ci/mmol was prepared by the reaction of [2-O-methyltyrosine]oxytocin with 2,2'-³H₂-acetanhydride. The analogue was stable in human pregnancy serum. Chymotrypsin split the Tyr(Me)-Ile and Leu-GlyNH₂ peptide bonds. In the presence of subcellular fractions of homogenates of the rat uterus and mammary gland, several metabolic products were formed from the analogue.

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The preparation and metabolic fate of tritiated Nα-acetyl[2-O-methyltyrosine]oxytocin - An inhibitor of the uterotonic action of oxytocin

Abstract

The preparation and metabolic fate of tritiated N^α-acetyl[2-O-methyltyrosine]oxytocin - An inhibitor of the uterotonic action of oxytocin