Collect. Czech. Chem. Commun.
1980, 45, 617-622
https://doi.org/10.1135/cccc19800617
Alpha- and beta-amylase immobilized by a covalent binding to hydroxyalkyl methacrylate gels
Karol Babor and Lubomíra Rexová-Benková
Institute of Chemistry, Slovak Academy of Sciences, 809 33 Bratislava
Abstract
Insoluble active preparation of alpha- and beta-amylase were prepared by a covalent binding of enzymes to hydroxyalkyl methacrylate gel (various kinds of Spheron type) activated with cyanogen bromide. The amount of the bound enzyme, its amylolytic activity on individual substrates, storage and heat stabilities, and pH-optimum were determined.
