Collect. Czech. Chem. Commun. 1981, 46, 1054-1058
https://doi.org/10.1135/cccc19811054

Isolation of plasma membranes from chick embryo chorioallantoic membranes and identification of main nucleolytic activities

Svetlana Pristašová

Institute of Virology, Slovak Academy of Sciences, 809 39 Bratislava

Abstract

The method of isolating plasma membranes from chick embryo chorioallantoic membranes is described along with some morphological and chemical properties of these membrane preparations. Besides 5'-nucleotidase, alkaline phosphodiesterase, alkaline phosphatase, ribonucleaseendonuclease and 2',3'-cyclic nucleotide 3'-phosphohydrolase activities, the plasma membranes possesses a high activity of 2',3'-cyclic nucleotide 2'-phosphohydrolase. They exert negligible activities for glucose 6-phosphatase and succinate dehydrogenase.