Collect. Czech. Chem. Commun.
1988, 53, 2890-2896
https://doi.org/10.1135/cccc19882890
New adsorbents for thymidylate synthase affinity chromatography
Krzysztof Pawełczaka, Leszek Krzyżanowskia, Barbara Rzeszotarskaa, Michał Kempnya, Piotr Wieczoreka, Joanna Cieslab and Wojciech Rodeb
a Institute of Chemistry, Pedagogical University of Opole, ul. Oleska 48, 45-052 Opole, Poland
b Nencki Institute of Experimental Biology, The Polish Academy of Sciences, ul. Pasteura 3, 02-093 Warszawa, Poland
Abstract
New affinity adsorbents, intended for chromatography of thymidylate synthase (EC 2.1.1.45) from different sources, consisting of p-[N-[(2-amino-4-hydroxy-6-quinazolinyl)-methyl]-N-2-propynylamino]benzoyl-γ-[α-(3-carboxypropylamino)]glutamyl-glutamyl immobilized either on macroporous copolymer of acrylonitrile and n-butyl acrylate or on macroporous polymer of acrylonitrile itself, both crosslinked with divinylbenzene and having aminoethyl groups, were obtained. Both adsorbents were found to be effective in dUMP-dependent binding of thymidylate synthase from regenerating rat liver.
