Collect. Czech. Chem. Commun.
1998, 63, 851-856
https://doi.org/10.1135/cccc19980851
Single-Step Separation of Lactate Dehydrogenase Using Thiophilic Chromatography
Milena Kmínková and Jiří Kučera
Food Research Institute, Radiová 7, CZ-102 31 Prague 10, Czech Republic
Abstract
Lactate dehydrogenase (EC 1.1.1.27) from carp (Cyprinus carpio) hepatopancreas was purified by the single-step chromatography on thiophilic sorbent. Hydrophobic negative sorption was used as negative adsorption step for final purification. Final specific activity was 8.88 U/mg protein and the yield 84%. The enzyme is not adsorbed during hydrophobic interaction chromatography, but proteolytic activity is adsorbed completely on hydrophobic sorbent. Thus hydrophobic adsorption can be used as a prepurification step.
Keywords: Lactate dehydrogenase; Carp; Thiophilic chromatography; Enzymes; Oxidoreductases; Cyprinus carpio.
