Collect. Czech. Chem. Commun.
2001, 66, 1219-1237
https://doi.org/10.1135/cccc20011219
Pulse Modulation - A Novel Approach to Gradient-Based Flow Injection Techniques
Armando Herbelin* and Jaromir Ruzicka
Department of Chemistry, University of Washington, 36 Bagley Hall, Box 351700, Seattle, WA 98195, U.S.A.
Abstract
Development of a novel system for generation of gradients in flow injection analysis by pulse modulation is described. These user-selectable gradients are created by computer-controlled mixing of two solutions with a total volume as low as 75 μl and can be delivered under incremental or continuous flow conditions. Applications such as automated, single-standard instrument calibration are expected to benefit from high-precision linear gradients (r2 = 0.99989, n = 55). Gradient methods in biochemisty and immunology such as kinetic measurement of biomolecular interactions will benefit from the small volume of these gradients, especially for analytes with limited availability.
Keywords: Flow injection analysis; FIA; Gradient; Pulse modulation; Analytical methods.
References: 17 live references.
