Collect. Czech. Chem. Commun.
2009, 74, 1675-1696
https://doi.org/10.1135/cccc2009108
Published online 2010-02-04 20:15:45
Polarographic and voltammetric study of genotoxic 2,7-dinitrofluoren-9-one and its determination using mercury electrodes
Vlastimil Vyskočil* and Jiří Barek
Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Hlavova 2030/8, CZ-128 43 Prague 2, Czech Republic
Abstract
Electrochemical behavior of genotoxic 2,7-dinitrofluoren-9-one was investigated by DC polarography and DC tast polarography, both at a dropping mercury electrode, and by cyclic voltammetry at a hanging mercury drop electrode, in buffered aqueous-methanolic solutions. The number of exchanged electrons was determined by constant-potential coulometry at a mercury pool electrode. A possible mechanism of the electrochemical reduction has been proposed. Optimal conditions were found for the determination of 2,7-dinitrofluoren-9-one by DC tast polarography in the concentration range from 2 × 10–6 to 1 × 10–5 mol l–1 and by differential pulse polarography (from 2 × 10–7 to 1 × 10–5 mol l–1), both at dropping mercury electrode, by differential pulse voltammetry (from 2 × 10–8 to 1 × 10–5 mol l–1) and by adsorptive stripping voltammetry (from 2 × 10–9 to 1 × 10–7 mol l–1), both at hanging mercury drop electrode. Practical applicability of the developed methods was verified on the direct determination of 2,7-dinitrofluoren-9-one in drinking water in 10–8 mol l–1 concentration range, and in drinking and river water (both in 10–9 mol l–1 concentration range) using preliminary separation and preconcentration by solid-phase extraction.
Keywords: 2,7-Dinitrofluoren-9-one; Nitro compounds; Mercury electrodes; Polarography; Voltammetry; Potentiostatic coulometry; Reduction mechanism; Electroanalysis; Solid-phase extraction; Electroreduction.
References: 39 live references.
